作者：Giri无糖爱情 ? Met al
译者：北医三院姚中强（yaozhongqiang@sina.com） 校正：孙琳
发布：柴静 审核：赵金霞
摘要：
引言:脑实质神经白塞病(NBD) 见于5%-15% 白塞病(BD) 患者，其特征是脑干和间脑结构的炎症。已经证实神经细胞凋亡参与了神经细胞丧失的过程。神经白塞患者血中检测出了抗神经元抗体。然而，这些抗体的致病性尚未研究过。
方法:为阐明血清抗体对神经元的潜在致病性，我们收集了7名神经白塞患者和7名健康对照的血清，并分为纯免疫球蛋白G (IgG) 和去除IgG的血清组分，而且，每一个组分都用来培养神经母细胞瘤细胞系SH-SY5Y。用毒性试验和末端转移酶介导的末端标记术(TUNEL)来评估细胞死亡。而且，使用实时定量PCR方法来评估数种凋亡相关标记物的表达。
结果:与其他处理相比书生阅读器 ，加入神经白塞患者IgG的培养的SH-SY5Y细胞死亡和凋亡均显著增加。 神经白塞患者的IgG也增加了细胞凋亡和存活通路主要分子的mRNA表达水平。
结论:我们的结果提示幽灵樟宜 ，来自神经白塞病患者的IgG可能通过凋亡机制而产生神经毒性。
附原文：Abstract INTRODUCTION: Parenchymal neuro-Beh?etdisease(NBD) isencountered in 5%-15% of Beh疯马夜总会 ?etdisease(BD) patients and is characterizedby inflammation of the brainstem and diencephalon structures. Neuronalapoptosis has been shown to participate in neuronal cell loss. Anti-neuronalantibodies have been identified in NBD patients. However, the pathogenicproperties of these antibodies have not been studied.METHODS:To delineate the potentialpathogenic activity of serum antibodies on neurons, pooled sera from seven NBDpatients and seven healthy controls were divided into purified immunoglobulin G(IgG) and IgG-depleted serum fractions, and each fraction was administered tocultured SH-SY5Y neuroblastoma cells. Cell death was evaluated with a toxicityassay and terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL)staining. Moreover, expression levels of several apoptosis markers wereevaluated with real time polymerase chain reaction (PCR).RESULTS:Administrationof NBD IgG to cultured SH-SY5Y cells induced significantly increased cell deathand apoptosis compared with other treatments. NBD IgG also enhanced the mRNAexpression levels of major apoptosis and cell survival pathway factors.CONCLUSION:Ourresults suggest that IgGs isolated from the sera of NBD patients have aneurotoxic activity that is presumably mediated by apoptotic mechanisms.
引自：Giri极品警察 ? M,Bireller S,Kü声声漫?ükali C?新娘和爸爸 ,Hana?asi H,傅小芸 De?irmencio?luS盼望造句 ,Tüzün E.Impact ofNeuro-Beh?etDiseaseImmunoglobulin G on Neuronal Apoptosis. Noro Psikiyatr Ars.2017 Mar;54(1):67-71.doi: 10.5152/npa.2016.19421. Epub 2016 Dec 8.